The Effect of Benzyl Amino Purine and 2,4-Dichlorophenoxyacetic Acid on in Vitro Propagation of Pranajiwa (Euchresta horsfieldii (Lesch) Benn.)

Abstract Pranajiwa (Euchresta horsfieldii (Lesch) Benn.) is an endangered medicinal plant known for its function as aphrodisiac, antidote, expectorant, and tonic. It also can be used to cure TBC. Research has found that pranajiwa difficult propagated, but the tissue culture propagation method never been applied this species. This research aimed figure out procedure induce callogenesis of with addition Benzyl Amino Purine (BAP) 2,4-Dichlorophenoxyacetic acid (2,4-D) determine proper combination BAP 2,4-D produce optimal callus. MS-based medium 25 combinations callus induction. Parameters observed in are appearing time, percentage inducted calluses, morphology observation, including texture color Result shows 90.67% calluses was successfully inducted. The obtained by combining 1 ppm 1,5 5 days friable green color.